Journal: Foods

Article Title: Chemical Composition Analysis of Highland Barley ( Hordeum vulgare L.) with Different Modification Methods and Lipid Metabolism Mechanism Analysis of Highland Barley with Microwave Fluidization Modification

doi: 10.3390/foods15081396

Figure Lengend Snippet: Protein expression levels ( A , H ) including ( B ) PPARγ, ( C ) Fabp4, ( D ) CD36, ( E ) SREBP-1c, ( F ) ADIPOQ, ( G ) SCD-1, ( I ) TLR4, ( J ) IKKβ, and ( K ) p-P65 in liver tissues among NCD, HFCD, and HFCD + HB-1. Data were expressed as mean ± SD ( n = 3) based on Dunnett’s test. ** indicated p < 0.01, *** indicated p < 0.001, **** indicated p < 0.0001.

Article Snippet: The specific antibody concentrations used in this study were as follows: anti-rabbit antibodies against Peroxisome proliferator-activated receptor γ (PPARγ) (1:1000, 58 kDa, Affinity, 16643-1-AP), FABP4 (1:1000, 15 kDa, Affinity, DF6035), CD36 (1:1000, 88 kDa, Affinity, DF13262), SREBP-1c (1:1000, 122 kDa, Affinity, AF6283), ADIPOQ (1:1000, 26 kDa, Affinity, DF7000), SCD-1 (1:1000, 41 kDa, Bioss, bs-3787R), p-P65 (1:1000, 65 kDa, Affinity, AF2006, Serine Ser536), TLR4 (1:1000, 100 kDa, Affinity, AF7017), IKKβ (1:1000, 87 kDa, Affinity, AF6010), and GAPDH (1:1000, 37 kDa, Xianzhi Biotech, AB-P-R 001).

Techniques: Expressing

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-A y mice.

doi: 10.1016/j.biopha.2021.111714

Figure Lengend Snippet: Fig. 9. GRO supplementation reduced the protein levels of SCD1 and PPARα and increased the level of phosphorylated AMPK in the liver. (A) Western blot analysis of SCD1 in the liver of 20-week-old mice. (B) Western blot analysis of PPARα in the liver of 20-week-old mice. (C) The ratio of phosphorylated AMPK to total AMPK levels in the liver of 20-week-old mice, as determined by Western blot analysis. Cont: control mice; GRO8: 0.8% GRO mice. The data were analyzed with Student’s t- test. *p < 0.05, *p < 0.01. Values are means ± SD (n = 5).

Article Snippet: After electrophoresis, the separated proteins were transferred to a polyvinylidene difluoride membrane (Immobilon, 0.2 μm pore, (Merck Millipore Burlington, MA, USA)) and incubated overnight at 4 ◦C with the following primary antibodies: polyclonal rabbit anti-mouse PPARα (1:3000, GeneTex, Los Angeles, CA, USA), β-actin (1:3000, GeneTex), total AMPKα1 (1:3000, GeneTex), phosphorylated AMPKα1 (Thr172) (1:1000, Cell Signaling Technology, Danvers, MA, USA), and SCD1 (1:1000, R&D Systems Minneapolis, MN, USA).

Techniques: Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: β-Sitosterol Suppresses Lipopolysaccharide-Induced Inflammation and Lipogenesis Disorder in Bovine Mammary Epithelial Cells

doi: 10.3390/ijms241914644

Figure Lengend Snippet: Antibody information and dilutions in this study.

Article Snippet: Rabbit anti- SCD polyclonal antibody , 1:1000 , bs-3787R , Bioss.

Techniques: